Emerald Lake is the most popular fishing destination in the park. Boat rentals are available at the Emerald Sports boathouse. Brook Char and Rainbow Trout are a frequent catch.
Cutthroat trout are highly sought after at Lake O'Hara, where visitor access is quite limited. Boat rentals are available at the Lake O'Hara Lodge.
Wapta Lake is easily accessible from the Trans Canada Highway on the east end of the park and offers good lake trout fishing. Sherbrooke Lake, a moderate 3km (2 mile) uphill hike whose trailhead starts opposite Wapta Lake, is also a great place to find lake trout.
Fly fishing is popular on the Kicking Horse River west of the Field townsite. Fishing is permitted from the confluence of the Kicking Horse and Yoho rivers downstream to the park boundary. Cutthroat and Rainbow Trout, Brook Char, Dolly Varden, and Whitefish are all common catches in the Kicking Horse River.